Antibody Humanization Using Monovalent Phage Display
نویسندگان
چکیده
منابع مشابه
Generation and characterization of a recombinant human CCR5-specific antibody. A phage display approach for rabbit antibody humanization.
We describe the isolation of a CCR5-specific antibody, ST6, from an antibody phage display library generated from an immune rabbit. ST6 was previously shown to efficiently prevent the surface expression of CCR5 when expressed intracellularly (Steinberger, P., Andris-Widhopf, J., Buhler, B., Torbett, B. E., and Barbas, C. F., III (2000) Proc. Natl. Acad. Sci. U. S. A. 97, 805-810). Because ST6 h...
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A critical factor in the successful isolation of new antibodies by phage display is the presentation of a correctly folded antigen. While this is relatively simple for soluble proteins which can be purified and immobilized onto a plastic surface, membrane proteins offer significant challenges for antibody discovery. Whole cell panning allows presentation of the membrane protein in its native co...
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The selection of antibodies from combinatorial libraries displayed on the surface of filamentous phage has become an important methodology for the generation of reagent, diagnostic, and therapeutic molecules and for the study of natural immune responses. Using this technique, antibody genes have been cloned from multiple species or expressed directly from large man-made repertoires of antibody-...
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inTrOducTiOn The production of human monoclonal mAbs for research and clinical use is closely related to the development of phage display technology, initially described by Smith in 1985 and further developed by other groups (e.g., Winter, McCafferty, Lerner, Barbas). Antibody phage display (APD) is based on genetic engineering of bacteriophages (viruses that infect bacteria) and repeated round...
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Antibody phage technology greatly facilitates the isolation of good-quality monoclonal antibodies to virtually any target antigen. Large combinatorial phage display libraries of human antibodies are routinely being used for the identification of antibody candidates for clinical applications. However, preclinical studies in rodents would benefit from the availability of good-quality single-pot m...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1997
ISSN: 0021-9258
DOI: 10.1074/jbc.272.16.10678